Establishment of callus culture of Melientha suavis Pierre

Establishment of the Shoot and Callus Culture of an Important Medicinal Plant Plumbago zeylanica

Medicinal plants are one of the important sources of natural products, which include essential oils, fragrances, pigments, feedstock, and pharmaceuticals. They are of great interest to the researches in biotechnology field as most of the pharmaceutical industries depend on them Chand et al. [1]. Plumbago zeylanica is an important medicinal plant which belongs to the Plumbaginaceae family. It is...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Callus culture and regeneration.

A simple technique for the establishment of nitrogenase in soybean callus culture.

A nitrogen-fixing association can be readily established in vitro between Rhizobium and cultured soybean root cells. Plant cells are grown as a thin callus on the surface of solid defined medium containing low levels of inorganic nitrogen and inoculated with bacteria during the active phase of growth. Acetylene reduction activities up to 275 nmoles ethylene/hr.g dry weight of cells have been re...

Virology / Virologie Establishment and maintenance of Soybean Mosaic Virus in soybean callus culture

Susceptibility to inoculation with Soybean mosaic virus (SMV) and virus activity were investigated in soybean callus cultures growing in vitro. Excised hypocotyls of susceptible soybean, Glycine max (L.) Merr. ‘Essex’, were cultured in Msoy medium in the light at 25 °C. Established calluses were inoculated with SMV in vitro by a soak–prick method. In addition, SMV-infected leaves of soybean ‘Le...